Laboratory Techniques with Reagents and Solutions

by Chavan, U D

ISBN: 9789390259625
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Imprint : Daya Publishing House
Year : 2021
Price : Rs. 14295.00
Biblio : 2018, viii+400p., figs., tbls., 25cm

Author Profile

Dr. U.D. Chavan obtained his M.Sc. (Ag) Biochemistry degree from Mahatma Phule Krishi Vidyapeeth, Rahuri in 1987. He received his Ph.D. degree in Food Science from Memorial University of Newfoundland St. John’s Canada in 1999. He has done International training on “Global Nutrition 2002” at Uppsala University Uppsala, Sweden in 2002. He also attended follow-up International workshop on “Global Nutrition 2002” at Hanoi, Vietnam in 2002. Dr. Chavan visited Denmark, Finland, Ireland, France, Switzerland, Poland, Spain, Vietnam, Thailand, England, and U.S.A. under “Global Nutrition 2002” Programme. Dr. Chavan worked as Senior Research Assistant in the Department of Biochemistry and Food Science and Technology at Mahatma Phule Krishi Vidyapeeth Rahuri from 1988 to 2000. During his Ph.D., he worked as Technician/Research Associate at Atlantic Cool Climate Crop Research Center and Agriculture and Agri-Food Canada. He received D.Sc. degree in 2006 from USA. He has guided 22 students for M.Sc. (Agri.) in Biochemistry and Food Science and Technology. From 2000 to 2004 he worked as an Assistant Professor of Biochemistry at Mahatma Phule Krishi Vidyapeeth Rahuri. Now, he is working as a Professor in the Department of Food Science and Technology, Senior Cereal Food Technologist in Sorghum Improvement Project and Foreign Student Advisor at Mahatma Phule Krishi Vidyapeeth, Rahuri, Dist, Ahmednagar, Maharashtra, India.

About The Book

This book contains most suitable procedure of laboratory techniques. It describes the basic laboratory rules, equipment and instrumentation, basic concepts, types of stock standard solutions, equivalent concentration, normality, normal solution, molar solution, molal solution, percent solution, parts per million (ppm), various types of buffer solutions, pH, spectrophotometry, atomic absorption spectrophotometry, chromatography viz. paper chromatography, thin layer chromatography (TLC), gas chromatography (GC), liquid chromatography (LC), gas-liquid chromatography (GLC), high performance liquid chromatography (HPLC), polymerase chain reaction (PCR), scanning electron microscope (SEM), radiation, enzyme assays and radioactivity and related calculations. These equipment and instruments are commonly used in practical laboratory. Important references have been also cited for detailed reading. It is hoped that the book is quite useful for students, teachers, researchers, biochemists, chemist, food chemist and person engaged in various laboratories.

Table of Contents

Preface v 1. Introduction Basic Laboratory Rules Basic Equipment and Instrumentation 1 2. Some Basic Concepts Scientific Notation Significant Figures Recording of Results Expressing Concentration of Solute Converting between Concentration Units Dilution of Concentration Solutions 41 3. Types of Stock Standard Solutions Introduction Ionic Standard Solution Element Standard Solution Formula Standard Solution 55 4. Equivalent Concentration Normal solutions Molar/Molarity solutions Molal solutions Percent solutions Parts Per Million (PPM Solution) 65 5. Buffer Solutions Principles Preparation Calculations 127 6. pH Acid and Bases Hydrogen ion Concentration and pH Measurement of pH 159 7. (i). Spectrophotometry Basic Principles Quantitative Applications (ii). Atomic Absorption Spectrophotometry Basic Principles Quantitative Applications 165 8. Chromatography Principles and Applications Paper Chromatography Thin Layer Chromatography (TLC) Gas Chromatography (GC) Liquid Chromatography (LC) Gas-Liquid Chromatography (GLC) High Performance Liquid Chromatography (HPLC) Polymerase Chain Reactions (PCR) Scanning Electron Microscope (SEM) Radiation 247 9. Enzyme Assays Principles and Applications Photometric Radiometric Electrochemical Assays Gel Electrophoresis 323 10. Radioactivity and related calculations 363 References 381 Appendices 399