Basic and Molecular Genetics

by Tomar, Arun Kumar, Archana T Tomar & Sukhvir Singh Tomar

ISBN: 9789387057937
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Imprint : Daya Publishing House
Year : 2018
Price : Rs. 17995.00
Biblio : xx+598p., figs., tbls., indx., 25cm

Author Profile

Dr. Arun Kumar Tomar is presently holding the position of Head, Division of Animal Genetics and Breeding for the last four year and Director ICAR-Central Sheep and Wool Research Institute, Avikanagar, via Jaipur, Rajasthan. He did Ph.D. (Animal Breeding) from CCS, HAU, Hisar, B.Sc. (Ag.) and M.Sc. (Ag.) in A.H. and Dairying from J. V. College, Baraut, Bagpat (U.P.). He has published few books and many research papers and has experience of editorial work in the capacity of Assistant Editor of Research Journals.<br/> Mrs. Archana Tomar is M.Sc., B.Sc. (Genetics), B.Ed. from Kurukshetra University, Kurukshetra (Haryana). She is presently engaged in teaching work, U.P. Govt. Education Department, Ghaziabad. She has 12 years experience of teaching work. <br/> Prof. Sukhvir Singh Tomar is well qualified (Ph.D.), retired Principal Scientist (AGB) from N.D.R.I.,Karnal (I.C.A.R.) having 40 years of research and teaching experience, has published about 300 research articles and 3 textbooks on population genetics and animal breeding. He has long experience for about 28 years as Editor of Research Journal and guided a number of M.Sc. and Ph.D. students.

About The Book

The present book is covered in 3 parts comprising 27 chapters. The first part covers “Mendelian or classical genetics” in 11 chapters dealing with introduction to genetics, sources of genetic variation, heredity and environment, Mendelism, Chromosomal theory of inheritance, Exceptions to Mendelism [Multiple alleles, gene expression (action) on phenotypic level, Linkage of genes and crossing over], Sex determination and sex controlled inheritance. The second part covers “Molecular genetics” in 14 chapters which includes genetics, genetic material (DNA) and its molecular structure, changes in its structure (mutation), and replication of DNA; Gene expression at molecular level (transcription and translation), Regulation of gene expression in prokaryotes and eukaryotes, Gene manipulation techniques and applications. The last part covers “Genetic Statistics” which includes 2 chapters on the probability and Chi-square testing of discrete variable. This book on “Genetics” has been written to fulfill the hope and requirement of post-graduate students, teachers and of those intend to appear and pass the competitive examinations on All India basis like SRF, NET and ARS conducted by ASRB (ICAR), UGC and UPSC.

Table of Contents

Preface vii Part 1: Mendelian Genetics 1. Introduction to Genetics 3 2. Variation in Populations 15 3. Heredity and Environment 33 4. Mendel’s Laws of Heredity 47 5. Chromosome Theory of Heredity 65 6. Multiple Alleles 71 7. Gene Action I. Allelic Interaction (Additive Gene Action) 87 8. Gene Action II. Non-Allelic Interaction (Epistasis) 101 9. Linkage and Crossing Over 121 10. Sex Determination 157 11. Sex Controlled Inheritance 171 Part 2: Molecular Genetics 12. Microbial Genetics: Bacteria and Viruses 191 13. Structure of Genetic Material: Nucleic Acids (DNA and RNA) 215 14. Mutation: General Aspects 255 15. Induction of Mutation 281 16. DNA Replication 297 17. Gene Expression – Molecular Mechanism (Protein Synthesis) I. Transcription (RNA Production) 317 18. Genetic Code 335 19. Gene Expression – Molecular Mechanism (Protein Synthesis) II. Translation (Polypeptide Production) 345 20. Gene Expression – Regulation I. Prokaryotes 351 21. Gene Expression – Regulation II. Eukaryotes 367 22. Gene Expression – Biochemical Pathway (Biochemical Genetics) 387 23. Techniques of Molecular Genetics – Recombinant DNA Technology 397 24. Applications of Molecular Genetics – Genome Manipulation 423 25. Markers and Genome Maps 439 Part 3: Genetic Statistics 26. Probability Rules 461 27. Chi-Square (?2) Test 493 Annexures A. 14.1 Mechanisms Involved in Transition Mutation 511 A. 14.2 Mechanism of Transposition 519 A. 15.1 Causes of Cancer: Genetic Disease of Cell Division 528 A. 19.1 Amino Acids: Structure and Polymerization 536 A. 19.2 Proteins Structure: Three-Dimensional Structure of Complex Molecule 540 A. 19.3 One Gene-One Polypeptide Concepts 543 A. 19.4 Roles of different Components in Protein Synthesis 545 A. 23.1 Biological Tools Used in Recombinant DNA Technology 550 A. 23.2 Restriction Endonuclease 554 A. 23.3 Plasmid, Phage or Viral Chromosome 559 A. 23.4 Sources and Procedures to obtain Isolated Gene 562 A. 23.5 Techniques of DNA Transfer (Integration) 570 A. 23.6 In situ Colony or Plaque Hybridization 573 A. 23.7 Amplification of DNA Sequence or Desired Gene In vitro: Polymerase Chain Reaction 576 A. 23.8 PROBE: Suitably Labelled Nucleic Acid Molecule of 15-30 Bases Long 581 A. 23.9 Autoradiography 585 A. 23.10 Electrophoresis 586 A. 23.11 Somatic Cell Hybridization 587 A. 23.12 Enzyme Linked Immune- Sorbent Assay (ELISA) 588 A. 23.13 Nick Translation 590 A. 23.14 Dot Blot Technique 591 Subject Index 593