Chromatography and Centrifugation Methods: A Beginner's Handbook

by Timir Tripathi

ISBN: 9789371708012
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Imprint : Daya Publishing House
Year : 2026
Price : Rs. 8990.00
Biblio : x+195p., figs., tabls., 23 cm

Author Profile

Dr. Timir Tripathi is a Professor of Molecular Biology in the Department of Zoology at North-Eastern Hill University (NEHU), Shillong. Previously, he served as the Regional Director of Indira Gandhi National Open University (IGNOU), Kohima. Prior to that role, he held the position of Senior Assistant Professor and Principal Investigator at the Department of Biochemistry, NEHU, Shillong. He holds a Ph.D. from Jawaharlal Nehru University, New Delhi, while conducting research at the Central Drug Research Institute, Lucknow. He has held visiting faculty positions at ICGEB, New Delhi, and Khon Kaen University, Thailand. With over 18 years of experience in teaching and research at the postgraduate and doctoral levels, he has made significant contributions to the understanding of protein structure, function, and dynamics. He has also developed and improved methods for investigating and analyzing proteins. As a principal investigator, he has successfully managed 12 research projects funded by national and international agencies. Dr. Tripathi has been honoured with numerous national and international awards and is a respected member of the National Academy of Sciences, India, and the Royal Society of Biology, UK. He has published over 100 research papers, reviews, and editorial articles in international journals. Additionally, he has edited five books and authored two textbooks for undergraduate and postgraduate students.

About The Book

"Chromatography and Centrifugation Methods: A Beginner's Handbook" is an essential guide for anyone studying or working in the fields of biochemistry, chemistry, biotechnology, life sciences, pharmaceuticals, environmental science, etc. This comprehensive handbook provides a thorough introduction to the principles, methods, and applications of chromatography and centrifugation methods. From the basic principles to the practical aspects of sample preparation, column selection, methodology, and optimization, this book covers it all. With clear explanations, it equips readers with the knowledge needed to perform chromatographic and centrifugal separations effectively. Whether you are a student, researcher, professional, or faculty, this book is your go-to resource for understanding and mastering these fundamental methods in chemical analysis. The b­ook provides a comprehensive overview of both chromatography and centrifugation methods, offering a holistic approach to the subject matter. It presents the principles and methods in a clear and accessible manner and simplifies complex concepts, making it easier for readers to grasp the fundamentals. With a focus on applications, the book highlights the use of the methods in various industries and scientific fields, showcasing their broad range of applications. The book includes a glossary of common terms to serve as a quick reference and resource for ­readers.

Table of Contents

Foreword v Preface vii 1. Introduction to Separation Methods 01 Introduction; Types of separation methods; Factors affecting separation; Applications of separation methods 2. Chromatography Methods 11 Introduction; Historical background; Basic principles of chromatographic methods; Classification of chromatographic methods; Types of chromatographic methods; Applications of chromatography 3. Paper Chromatography 21 Introduction; Principles; Types of paper chromatography; Methodology; Applications 4. Thin-Layer Chromatography 31 Introduction; Principles; Types of TLC; Methodology; Applications 5. Ion-Exchange Chromatography 41 Introduction; Principles of IEC; Types of IEC; Methodology; Factors affecting IEC; Techniques and optimization strategies; Applications; Advancements and innovations in IEC 6. Gel Filtration Chromatography 59 Introduction; Principles; Methodology; Factors affecting GFC;. Types of matrices in GFC; Important parameters in GFC; Applications 7. Affinity Chromatography 75 Introduction; Principles; Resins in affinity chromatography; Methodology; Types of affinity chromatography; Applications x 8. Hydrophobic Interaction Chromatography 93 Introduction; Principles; Types of HIC; Methodology; Applications; Reverse phase chromatography; Comparison of HIC and RPC 9. Gas Chromatography 103 Introduction; Principles; Types of GC; Instrumentation; Applications 10. High-Performance Liquid Chromatography 115 Introduction; Principles; Types of HPLC; HPLC column selection and optimization; Instrumentation; Fast-pressure liquid chromatography; Difference between HPLC and FPLC; Applications 11. Other Chromatography Methods 131 Chiral chromatography; Countercurrent chromatography; Electrochromatography; Flash chromatography; Hydrophilic interaction chromatography; Hydroxyapatite chromatography; Multi-dimensional chromatography; Pyrolysis gas chromatography; Supercritical fluid chromatography 12. Centrifugation Methods 139 Introduction; Historical background; Basic principles of centrifugation; Types of centrifugation methods; Centrifuge design and operation; Sample preparation for centrifugation; Advancements and innovations in centrifugation methods; Applications 13. Other Separation Methods 161 Filtration; Distillation; Extraction; Evaporation; Crystallization; Precipitation; Electrolysis; Solvent extraction Glossary of Common Terms 185 Index 193