Introduction to Spectroscopic Methods

by Timir Tripathi

ISBN: 9789371704632
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Imprint : Daya Publishing House
Year : 2026
Price : Rs. 2490.00
Biblio : xii+158p.

Author Profile

Dr. Timir Tripathi is the Regional Director of Indira Gandhi National Open University (IGNOU), Regional Centre Kohima, Nagaland, India. Earlier, he served as a Senior Assistant Professor at the Department of Biochemistry, North-Eastern Hill University, Shillong, India. He holds a Ph.D. from the Central Drug Research Institute, Lucknow, India. He was a visiting faculty at ICGEB, New Delhi, India (2011), and Khon Kaen University, Thailand (2015). He is known for his research in protein biophysics, biochemistry, structural biology, and drug discovery. He has over 17 years of experience in teaching and research on protein structure, function, and dynamics at the post-graduate and doctoral levels. He has developed and improved methods to investigate and analyze proteins. He has handled 12 research projects as a principal investigator from various national and international funding agencies. He has received several national and international awards. He is an elected member of the National Academy of Sciences, India, and the Royal Society of Biology, UK. He has published more than 100 research papers, reviews, commentaries, viewpoints, and editorial articles in international journals, has edited four books, and published several book chapters. “An exceptional, concise, and up-to-date textbook for Indian students that provides a complete understanding of spectroscopy. The concepts are explained in very simple language. This book will be useful to both undergraduate and postgraduate students” Prof. T.P. Singh, DST-SERB Distinguished Professor, AIIMS, New Delhi “This book includes the basic theory, instrumentation and possible applications of the spectroscopic methods in very clear and easily understandable terms. I congratulate the author for his commitment and effort in bringing all important techniques together in this book” Prof. K.R.S. Sambasiva Rao, Vice-Chancellor, Mizoram University, Aizawl

About The Book

Spectroscopic techniques are used in various branches of modern biology, life sciences, chemistry, physics, etc. A clear understanding of the underlying principles is vital to learning their applications. This book presents a series of lecture notes on various spectroscopic methods used in biological and chemical sciences. It contains chapters written in simple language with clear illustrations/figures that the students can easily understand. After going through the chapters, the students will get an idea of the fundamentals and principles of spectroscopic methods for their applications in biochemistry, biotechnology, molecular biology, medicinal chemistry, pharmacology, pharmaceutics, biophysics, and structural biology. Key Features: It contains concise topics written in simple words in the form of class notes, so the students can easily understand the covered techniques. It provides an in-depth understanding of the theory behind the techniques, with each chapter discussing the basics, principles, associated concepts, and applications of the method. It will serve as a valuable guide for students of bachelor's, master's and bachelors to doctoral degrees.

Table of Contents

Foreword v Preface vii 1. Fundamental Principles of Spectroscopy 1 1. Introduction 2. Basic Features of Light 3. Electromagnetic Spectrum 4. Interaction of Light with Matter 5. Atomic/Molecular Processes Associated with Light 6. Basics of Electronic Transitions 7. General Construction of a Spectrometer 8. Physical Limitations of a Spectroscopic Measurement 2. Ultraviolet/Visible Spectroscopy 15 1. Introduction 2. Chromophores 3. Transmittance and Absorbance 4 Principle of UV/Vis Spectroscopy-Beer-Lambert’s Law 5. Construction of a Spectrophotometer 6. Types of Spectrophotometers 7. Application of UV/Vis Spectroscopy x 3. Fluorescence Spectroscopy 33 1. Introduction 2. Principle of Fluorescence Spectroscopy 3. Important Terminologies in Fluorescence Spectroscopy 4. Effect of Solvents on Fluorescence Spectra 5. Characteristics of the Fluorescence Phenomenon 6. Fluorophores 7. Construction of a Fluorescence Spectrometer 8. Applications of Fluorescence Spectroscopy 4. Circular Dichroism Spectroscopy 55 1. Introduction 2. Plane Polarized Light 3. Circularly Polarized Light 4. Principle of Circular Dichroism Spectroscopy 5. Construction of a CD Spectrometer 6. CD of Biomolecules 7. Applications of CD spectroscopy 5. Infra-red Spectroscopy 69 1. Introduction 2. Molecular Vibrations 3. Absorption of IR Radiation 4. Characteristics of IR Spectra 5. Construction of an IR Spectrometer 6. Applications of IR Spectroscopy 6. Raman Spectroscopy 79 1. Introduction 2. Scattering of Light 3. Origin of Raman Spectra 4. Principle of Raman Scattering 5. Construction of a Raman Spectrometer 6. Improving Raman Signals 7. Applications of Raman Spectroscopy 8. Differences between IR and Raman Spectroscopy xi 7. Nuclear Magnetic Resonance Spectroscopy 95 1. Introduction 2. Principle of NMR Spectroscopy 3. Working of an NMR Spectroscopy Experiment 4. 13C NMR Spectroscopy 5. 1H NMR Spectroscopy 6. Example of a 13C and 1H Spectra 7. Applications of NMR 8. X-ray Spectroscopy 111 1. Introduction 2. Properties of X-rays 3. Steps in X-ray Crystallography 4. Construction of an X-ray Spectrometer 5. Working of an X-ray Diffractometry Experiment 6. Applications of X-ray Spectroscopy 7. Comparison of X-ray Crystallography and NMR Spectroscopy 9. Electron Spin Resonance Spectroscopy 121 1. Introduction 2. Principle of ESR Spectroscopy 3. Construction of an ESR spectrometer 4. Applications of ESR Spectroscopy 10. Atomic Absorption Spectroscopy 125 1. Introduction 2. Principle of Atomic Absorption Spectroscopy 3. Steps in AAS 4. Instrumentation 5. Experimental Procedures 6. Applications of AAS 11. Turbidimetry and Nephelometry 131 1. Introduction 2. Turbidimetry 3. Nephelometry xii 4. Factors Affecting Light Scattering 5. Applications of Turbidimetry and Nephelometry 12. Mass Spectrometry 135 1. Introduction 2. Principle of Mass Spectrometry 3 Construction of a Mass Spectrometer 4. Types of Mass Spectrometers 5. Mass Spectrometry Coupled with Chromatography 6. Protein Sequence Identification by Mass Spectrometry 7. Applications of Mass Spectrometry 8. Difference between NMR Spectroscopy and Mass Spectrometry Index 155