Molecular Techniques in Crop Improvement

by Phundan Singh

ISBN: 9789359193748
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Imprint : Daya Publishing House
Year : 2025
Price : Rs. 7395.00
Biblio : xii+198p., tabls., figs., col., ind 25 cm

Author Profile

Dr. Phundan Singh hails from a reputed agricultural family of Western Uttar Pradesh [Village-Sakauti, Tehsil- Mawana, District- Meerut]. He graduated in Agriculture from Agra University, Agra and obtained his Master and Doctorate degrees from Kanpur University, Kanpur. He has throughout a brilliant academic record. His area of specialization is Plant Breeding and Genetics. Dr. P. Singh has total experience of 44 years in the field of Genetics and Plant Breeding. He has served the Central Institute for Cotton Research, Nagpur for about 32 years in various capacities such as Scientist, Senior Scientist, Principal Scientist, Head of Division and Director. He has 300 publications to his credit. He has participate and presented 80 research papers in various National and International Seminars, Symposia and Conferences He has authored 88 books on Genetics and Plant Breeding. He has also authored 18 technical bulletins on various aspects of cotton and contributed 13 chapters in various books. Dr P. Singh is a member of several Scientific Societies, referee of different journals, Expert member of Selection Committees and M. Sc. and Ph. D. Examiner in different Agricultural Universities. The Institute was recognized as the referral Laboratory for detection of Bt gene and received the best Annual Report Award when he was the Director. He has visited several countries such as Russia, Belarus, Ukraine, Crimea, Uzbekistan, Canada, USA, England, Austria and Germany.

About The Book

Present book has been designed to provide comprehensive information related to Molecular Techniques in one compact volume of 20 chapters. This book would be useful to the students, teachers and researchers engaged in the field of Genetics, Plant Breeding and Biotechnology.

Table of Contents

Preface vii 1. Introduction to Molecular Techniques 1 Introduction – Gel Electrophoresis – Polymerase Chain Reaction – Gene Cloning – DNA Sequencing – Southern Blotting – Northern Blotting – Western Blotting – Gene Silencing – DNA Fingerprinting – DNA Micro Arrays – Fluorescence in situ Hybridization – Gene Mapping – Allele Specific Oligonucleotide – Summary – Questions. 2. DNA Isolation 9 Introduction – Extraction of Onion DNA – Extraction of Wheat Germ DNA – Isolation of DNA Fragments – Gene Detection – Summary – Questions. 3. Gene Cloning 17 Introduction – Steps Involved – Gene Libraries – Construction of Library – Advantages of Gene Cloning – Limitations of Gene Cloning – Application in Agriculture – Summary – Questions. 4. Polymerase Chain Reaction (PCR) 25 Introduction – Steps Involved – Applications of PCR – Advantages and Disadvantages of PCR – Summary – Questions. 5. Gene Detection Techniques 31 Introduction – Gel Electrophoresis – Southern Blotting – Northern Blotting – Western Blotting – Probes for DNA Detection – Summary – Questions. 6. Genotyping and Phenotyping 37 Introduction – Comparison of Genotype and Phenotype – Genotyping – Phenotyping – Comparison of Genotyping and Phenotyping – Summary – Questions. 7. Quantitative Trait Locus (QTL) Mapping 43 Introduction – QTL Mapping – Future Thrusts – Summary – Questions. 8. DNA Fingerprinting 49 Introduction – Methods of DNA Fingerprinting – Types of DNA Fingerprinting – Steps Involved – Advantages – Drawbacks – Applications in Crop Improvement – Summary – Questions. 9. Methods of Foreign Gene Transfer 57 Introduction – Types of Gene Transfer – Steps Involved – Methods of Foreign Transfer – Summary – Questions. 10. Molecular Markers 65 Introduction – Types of Marker – Properties of an Ideal DNA Marker – Types of DNA Markers – Applications in Crop Improvement – Summary – Questions. 11. Marker Assisted Selection 79 Introduction – Main Features – Marker Assisted Selection – Gene versus Marker – Selection for Major Gene Linked to Marker – Situation for Use of MAS – Steps Involved in MAS – Applications of Markers – Advantages of MAS – Disadvantages of MAS – Summary – Questions. 12. Transgenic Breeding 87 Introduction – Transgenic Plants – Advantages of Transgenic Technology – Steps Involved – Transgenic vs Conventional Breeding – Applications – Practical Achievements – Probable Risks – Future Scope – Summary – Questions. 13. Transgenic Male Sterility 97 Introduction – Transgenic Male Sterility – Antisense Gene Approach – Summary – Questions. 14. Mapping Populations 107 Introduction – Recombinant Inbred Lines [RILS] – Near Isogenic Lines [NILs] – F2 Populations – Backcross Populations – Bulk Segregant Analysis [BSA] – Doubled Haploids – Summary – Questions. 15. Introduction to Genomics 113 Introduction – Types of Genomics – Classification – Proteomics – Metabolomics – Genomics in Crop Plants – Genome Mapping in India – Gene to be Mapped – Some Genome Mapping Laboratories – Applications in Crop Improvement – Achievements – Limitations – Future Thrusts – Summary – Questions. 16. Introduction to Bioinformatics 123 Introduction – Types of Bioinformatics – Applications in Plant Breeding – Summary – Questions. 17. Gene Revolution 131 Introduction – Main Features of Gene Revolution – Green Revolution and Gene Revolution – Advantages of GM Technology – The Future of Gene Technology – Summary – Questions. 18. Nanotechnology in Crop Improvement 141 Introduction – Main Features – Brief History – Applications – Applications in Crop Improvement – Achievements – Application in Agriculture – Applications in Other Fields – Implications of Nanotechnology – Summary – Questions. 19. Reverse Breeding [RB] 151 Introduction – Important Features – Steps Involved – Forward and Reverse Breeding – Practical Applications – Reverse Breeding and Marker Assisted Breeding – Limitations – Doubled Haploids – Future Scope – Summary – Questions. 20. Space and Speed Breeding 159 Introduction – A. Space Breeding – B. Speed Breeding – Summary – Questions. Appendices 169 Glossary 183 Some Useful References 191 Key References 193 Books by Dr. Phundan Singh 195