Principles and Methods in Plant Biology

by Pullaiah, T

ISBN: 9789354615627
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Imprint : Daya Publishing House
Year : 2023
Price : Rs. 7295.00
Biblio : xii+194 p., figs., tabls., ind., 25 cm

Author Profile

Dr. Ch Mohan is currently working as a research assistant. He has done M.Sc., B.Ed., and Ph.D., from Osmania University, Hyderabad, Telangana, India. He has published many research papers in National and International Journals. He presented poster and oral presentation in many conferences in the area of Botany. His Areas of specialization is plant biotechnology, molecular genetics and plant biochemistry. Dr. J.M. Upendra is currently working as a research assistant. He has done M.Sc., from Andhra University, Andhra Pradesh, India. He was awarded Ph.D., in Botany from Jai Narain Vyas University, Jodhpur, Rajasthan, India in the year 2017. He has published many research papers in International Journals. He presented many poster and oral presentations in National and International conferences in the area of plant science. His Areas of specialization is plant breeding, biochemical and molecular studies. Prof. T. Pullaiah obtained his M. Sc. (1973) and Ph. D. (1976) degrees in Botany from Andhra University. He was a Post-doctoral Fellow at Moscow State University, Russia, during 1976-1978. He travelled widely in Europe and visited Universities and Botanic Gardens in about 17 countries. He joined Sri Krishnadevaraya University as Lecturer (1979) and became Professor (1993). He held several positions in the University as Dean, Faculty of Life Sciences; Head, Department of Botany; Chairman, Board of Studies in Botany; Head, Department of Sericulture; Co-ordinator and Chairman, Board of Studies in Biotechnology; Principal, Sri Krishnadevaraya University College. He retired from active Service in 2011 and was selected by UGC as UGC-BSR Faculty Fellow in the Department of Botany, Sri Krishnadevaraya University. He has published 75 books, 335 research papers and 35 popular articles. His books include Flora of Andhra Pradesh (5 volumes), Biodiversity in India (8 volumes). Flora of Telangana (3 volumes), Encyclopaedia of World Medicinal Plants (5 volumes), Ethnobotany of India (5 volumes), Global Biodiversity (4 volumes), Orchids of Eastern Ghats and Monograph on Brachystelma and Ceropegia in India. He was the Principal-Investigator of 20 major Research Projects totalling more than a Crore of Rupees funded by DBT, DST, CSIR, UGC, BSI, WWF, GCC etc. Under his guidance, 54 students obtained their Ph. D. degrees and 34 students their M. Phil. Degrees. He is the recipient of Best Teacher Award from the Government of Andhra Pradesh, Prof. P. Maheshwari Gold Medal and Dr. G.Panigrahi Memorial Award of Indian Botanical Society and Prof. Y. D. Tiagi Gold Medal of the Indian Association for Angiosperm Taxonomy. He was President of Indian Association for Angiosperm Taxonomy (2013) and President of the Indian Botanical Society (2014). He was a member of Species Survival Commission of International Union for Conservation of Nature and Natural Resources (IUCN).

About The Book

Plant biology has emerged as most important addition to conventional crop improvement and in research laboratory programmes. However, advanced techniques to study and work in the field of plant biology existed only from few decades. Our manual “Principles and Methods in Plant Biology” has been presented in a manner that will allow even a novice reader appreciate and understand the theory and perform advanced protocols and techniques in plant biology. Various chapters in this manual include information pertaining to fractionalization procedures, separation procedures, estimation procedures, staining procedures, tissue culture procedures, genetic marker procedures, DNA markers types and their development, markers assisted selection, basic plant biology manipulations, biochemical and phytochemical analysis information etc. We hope that following these working protocols and understanding the theoretical bases behind them will benefit your research.

Table of Contents

Preface v Abbreviations xi 1. Good Laboratory Practices 1 2. Fractionalization Procedures 5 Fractionalization of Cell Components 6 Isolation of Genomic DNA 9 Isolation of Chloroplasts 14 Isolation of Mitochondria 16 3. Separation Procedures 19 Chromatography 20 Thin Layer Chromatography 24 Column Chromatography 32 Electrophoresis 36 Polyacrylamide Gel Electrophoresis 40 4. Estimation Procedures 47 Estimation of Total Free Amino Acids 48 Estimation of Proline 51 Estimation of Protein 53 Estimation of Amylases 58 Estimation of Glutamate Synthase 61 Estimation of Glutamine Synthetase 64 Estimation of Malate Dehydrogenase 67 Estimation of Nitrate Reductase 70 Estimation of Nitrite reductase 73 Estimation of Peroxidase 76 Color Reactions of Carbohydrates 79 Estimation of Total Carbohydrates 83 Estimation of Reducing Sugars 87 Estimation of Starch 92 Estimatoin of Amylose 95 Estimation of Glucose 98 Estimation of Fructose 101 Estimation of Cellulose 104 Estimation of Crude Fiber 107 Estimation of Oil in Oilseed 109 Estimation of Free Fatty Acids 111 Estimation of DNA 113 Estimation of RNA 119 Estimation of Ascorbic Acid 122 Estimation of Phenols 125 Estimation of Tannins 127 Estimation of Phytic Acid 131 Estimation of Cyanogens 134 Estimation of Gossypol 137 Estimation of Trypsin Inhibitor 140 5. Staining Procedures 143 Cell Division Staining 144 Cytochemical Staining 148 6. Tissue Culture Procedures 153 Nutrient Medium 154 Micropropagation 162 Callus Culture 166 Seed Culture 169 Embryo Culture 172 Anther Culture 175 Organ Culture 178 Protoplast Culture 181 7. Genetic Marker Procedures 185 Index 193