Laboratory Manual of Biochemistry
by Nawaz Ahmad Khan
ISBN: 9789351304302
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Imprint : Daya Publishing House
Year : 2021
Price : Rs. 4995.00
Biblio : viii+105p.,figs.,tabls.,ind., 25 cm
Author Profile
"Dr. Nawaz Ahmad Khan did his B.Sc. (Ag) and M.Sc. (Ag) Biochemistry from NDUA&T, Kumarganj Faizabad in year 1988 and 1991 respectively. He got Vice Chancellor Gold Medal at M.Sc. (Biochemistry). He was awarded his Ph.D. (Biochemistry) from Gujarat Agricultural University, Junagarh in year 2000. Dr. Khan has to his credit more than 24 scientific publications and review articles which have appeared in international recognized journals. He guided M.Sc. (Ag.) Biotechnology and Ph.D. students. His research areas include Biotic Stress in Plants and Molecular Biology. He is recipient of ‘Young Scientist Award’ 2013 by Alumni Association of NDUA&T Kumarganj. Currently he is working as Assistant Professor, Department of Biotechnology, NDUA&T, Kumarganj, Faizabad. <BR> Dr. K.N. Singh did M. Phil. from JNU, New Delhi and obtained Ph.D. degree from Cambridge University, U.K. in 1990. He started his carrier as Research Associate at TERI, New Delhi and then joined Tamil Nadu Agricultural University, Coimbatore as Assistant Professor. In early 2005 he joined NDUAT as Professor in Biotechnology department. At present he is working as Head of the Departments of Biotechnology and Biochemistry. He also served as Visiting Scientist at IRRI, Philippines funded by Rockefeller Foundation. He has guided M.Sc. and Ph.D. students. Dr. Singh has published 33 research papers and review articles in journals of repute. Dr. Singh has successfully complete a number research projects funded of Govt. of India. Dr. Singh has participated delivered lectures at various National and International conferences/symposium."
About The Book
"The book ‘Laboratory Manual of Biochemistry’ primarily designed for undergraduate and postgraduate Students of Biochemistry, Horticulture and Biotechnology,the book also be useful to professionals, researchers and entrepreneurs. This practical loboratory manual has been designed to familiarise students with such protocols with flow chart that can be understood easily. <BR> KEY FEATURES <BR> * Written in easy to understand style. <BR> * Provides simple clear and authoritative guide to the principles and scope of Biochemistry."
Table of Contents
Preface v <BR> 1. Estimation of Titratable Acidity 1 <BR> 2. Estimation of Ascorbic Acid (Vitamin C) 3 <BR> 3. Determination of Chlorophyll 6 <BR> 4. Determination of Carotene (Vitamin A) 10 <BR> 5. Separation and Determination of Carotenoids 14 <BR> 6. Estimation of Total Soluble Sugar 18 <BR> 7. Estimation of Reducing Sugar 21 <BR> 8. Determination of Total Phenols 24 <BR> 9. Estimation of True Protein by Folin – Lowry’s Method 27 <BR> 10. Determination of Methionine 30 <BR> 11. Estimation of Proline 33 <BR> 12. Determination of Tryptophan 35 <BR> 13. Estimation of Pectin Substances 37 <BR> 14. Determination of Furfural 42 <BR> 15. Determination of Stevens Clouds Stability Test 45 <BR> 16. Determination of Ethanol 47 <BR> 17. Determination of Acetaldehyde (CH3CHO) 50 <BR> 18. Carotenoids Estimation by HPLC 53 <BR> 19. Sugar Analysis by HPLC 56 <BR> 20. Organic Acid Determination by HPLC 59 <BR> 21. Determination of Thiabendazole in Fruit by UV <BR> Spectrophotometer 61 <BR> 22. Determination of Peroxidase Enzyme (Donor : H2O2 <BR> Oxidoreductase EC 1.11.17) 67 <BR> 23. Determination of Polyphenol Oxidase Enzyme (PPO) <BR> (Monophenol, Dihydroxy Phenylalanine Oxygen <BR> Oxidoreducaste EC 1.14.18.1) 70 <BR> 24. Determination of Catalase Enzyme <BR> (Hydrogen Peroxide : Hydrogen Peroxide Oxidoreductase <BR> EC 1.11.1.6) 72 <BR> 25. Isolation of Plant Genomic DNA by C-Tab Method 75 <BR> 26. SDS-PAGE Electrophoresis of Protein 82 <BR> Annexure-I 89 <BR> Annexure-II 93 <BR> Annexure-III 96 <BR> Annexure-IV 98 <BR> Annexure-V 99 <BR> Annexure-VI 101 <BR> Index 103
